Synopsis

This study investigated sebocyte differentiation and lipid metabolism in acne vulgaris using the SEBO662AR cell line, a physiologically relevant in vitro model characterized by stable expression of a functional androgen receptor. The research aimed to evaluate its utility as a tool for advanced skin care testing.
Acne vulgaris is associated with irregular sebum production influenced by hormonal fluctuations, changes in the microbiome, and dietary factors. The study employed a differentiation mixture (Diff Mix) to simulate these imbalances and analyzed gene expression and lipid synthesis. The results revealed significant changes in gene expression related to sebocytes, as well as a notable increase in squalene accumulation, indicating the functionality of squalene epoxidase (SQLE). Importantly, the alterations induced by Diff Mix were reversible with specific inhibitors.
This work highlights the SEBO662AR cell line’s value as a model for acne-related research. By modeling pathological sebum production, it serves as a suitable platform for studying lipid synthesis mechanisms, offering significant potential for skin care testing and product development targeting sebaceous activity.